PIF1 5'-to-3' DNA helicase

Protein-coding gene in the species Homo sapiens
PIF1
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

5FHH

Identifiers
AliasesPIF1, C15orf20, PIF, PIF1 5'-to-3' DNA helicase
External IDsOMIM: 610953; MGI: 2143057; HomoloGene: 99775; GeneCards: PIF1; OMA:PIF1 - orthologs
Gene location (Human)
Chromosome 15 (human)
Chr.Chromosome 15 (human)[1]
Chromosome 15 (human)
Genomic location for PIF1
Genomic location for PIF1
Band15q22.31Start64,815,632 bp[1]
End64,825,668 bp[1]
Gene location (Mouse)
Chromosome 9 (mouse)
Chr.Chromosome 9 (mouse)[2]
Chromosome 9 (mouse)
Genomic location for PIF1
Genomic location for PIF1
Band9|9 CStart65,587,160 bp[2]
End65,595,967 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • ventricular zone

  • gonad

  • ganglionic eminence

  • stromal cell of endometrium

  • mucosa of transverse colon

  • appendix

  • granulocyte

  • bone marrow cells

  • spleen

  • lymph node
Top expressed in
  • otic vesicle

  • hand

  • Paneth cell

  • ventricular zone

  • primitive streak

  • tail of embryo

  • genital tubercle

  • endothelial cell of lymphatic vessel

  • hair follicle

  • thymus
More reference expression data
BioGPS
n/a
Gene ontology
Molecular function
  • nucleotide binding
  • DNA binding
  • telomeric DNA binding
  • helicase activity
  • DNA helicase activity
  • single-stranded DNA helicase activity
  • 5'-3' DNA/RNA helicase activity
  • hydrolase activity
  • ATP binding
  • magnesium ion binding
  • telomerase inhibitor activity
  • 5'-3' DNA helicase activity
  • G-quadruplex DNA binding
Cellular component
  • replication fork
  • mitochondrion
  • nucleus
Biological process
  • DNA recombination
  • regulation of telomere maintenance
  • negative regulation of telomerase activity
  • G-quadruplex DNA unwinding
  • cellular response to DNA damage stimulus
  • telomere maintenance
  • DNA repair
  • DNA duplex unwinding
  • mitochondrial genome maintenance
  • DNA replication
  • negative regulation of telomere maintenance via telomerase
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

80119

208084

Ensembl

ENSG00000140451

ENSMUSG00000041064

UniProt

Q9H611

Q80SX8

RefSeq (mRNA)

NM_001286496
NM_001286497
NM_001286499
NM_025049

NM_172453
NM_001357526
NM_001357527

RefSeq (protein)

NP_001273425
NP_001273426
NP_001273428
NP_079325

NP_766041
NP_001344455
NP_001344456

Location (UCSC)Chr 15: 64.82 – 64.83 MbChr 9: 65.59 – 65.6 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

PIF1 5'-to-3' DNA helicase is a protein that in humans is encoded by the PIF1 gene. [5]

Function

This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013].

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000140451 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000041064 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ "Entrez Gene: PIF1 5'-to-3' DNA helicase". Retrieved 2018-05-17.

Further reading

  • Zhang DH, Zhou B, Huang Y, Xu LX, Zhou JQ (2006). "The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity". Nucleic Acids Res. 34 (5): 1393–404. doi:10.1093/nar/gkl029. PMC 1390689. PMID 16522649.
  • Mateyak MK, Zakian VA (December 2006). "Human PIF helicase is cell cycle regulated and associates with telomerase". Cell Cycle. 5 (23): 2796–804. doi:10.4161/cc.5.23.3524. PMID 17172855.
  • Futami K, Shimamoto A, Furuichi Y (September 2007). "Mitochondrial and nuclear localization of human Pif1 helicase". Biol. Pharm. Bull. 30 (9): 1685–92. doi:10.1248/bpb.30.1685. PMID 17827721.
  • Gu Y, Masuda Y, Kamiya K (November 2008). "Biochemical analysis of human PIF1 helicase and functions of its N-terminal domain". Nucleic Acids Res. 36 (19): 6295–308. doi:10.1093/nar/gkn609. PMC 2577353. PMID 18835853.
  • George T, Wen Q, Griffiths R, Ganesh A, Meuth M, Sanders CM (October 2009). "Human Pif1 helicase unwinds synthetic DNA structures resembling stalled DNA replication forks". Nucleic Acids Res. 37 (19): 6491–502. doi:10.1093/nar/gkp671. PMC 2770657. PMID 19700773.
  • Sanders CM (August 2010). "Human Pif1 helicase is a G-quadruplex DNA-binding protein with G-quadruplex DNA-unwinding activity". Biochem. J. 430 (1): 119–28. doi:10.1042/BJ20100612. PMID 20524933.
  • Gagou ME, Ganesh A, Thompson R, Phear G, Sanders C, Meuth M (July 2011). "Suppression of apoptosis by PIF1 helicase in human tumor cells". Cancer Res. 71 (14): 4998–5008. doi:10.1158/0008-5472.CAN-10-4404. PMID 21616935.
  • Chisholm KM, Aubert SD, Freese KP, Zakian VA, King MC, Welcsh PL (2012). "A genomewide screen for suppressors of Alu-mediated rearrangements reveals a role for PIF1". PLOS ONE. 7 (2): e30748. Bibcode:2012PLoSO...730748C. doi:10.1371/journal.pone.0030748. PMC 3276492. PMID 22347400.
  • Ramanagoudr-Bhojappa R, Chib S, Byrd AK, Aarattuthodiyil S, Pandey M, Patel SS, Raney KD (May 2013). "Yeast Pif1 helicase exhibits a one-base-pair stepping mechanism for unwinding duplex DNA". J. Biol. Chem. 288 (22): 16185–95. doi:10.1074/jbc.M113.470013. PMC 3668774. PMID 23596008.

External links

  • Overview of all the structural information available in the PDB for UniProt: Q9H611 (ATP-dependent DNA helicase PIF1) at the PDBe-KB.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.


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