DPH5

Protein-coding gene in the species Homo sapiens
DPH5
Identifiers
AliasesDPH5, CGI-30, HSPC143, NPD015, AD-018, diphthamide biosynthesis 5
External IDsOMIM: 611075; MGI: 1916990; HomoloGene: 6471; GeneCards: DPH5; OMA:DPH5 - orthologs
Gene location (Human)
Chromosome 1 (human)
Chr.Chromosome 1 (human)[1]
Chromosome 1 (human)
Genomic location for DPH5
Genomic location for DPH5
Band1p21.2Start100,989,623 bp[1]
End101,026,088 bp[1]
Gene location (Mouse)
Chromosome 3 (mouse)
Chr.Chromosome 3 (mouse)[2]
Chromosome 3 (mouse)
Genomic location for DPH5
Genomic location for DPH5
Band3|3 G1Start115,887,837 bp[2]
End115,934,361 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • body of pancreas

  • gonad

  • left ovary

  • buccal mucosa cell

  • right adrenal gland

  • ganglionic eminence

  • right adrenal cortex

  • right ovary

  • tibialis anterior muscle

  • ventricular zone
Top expressed in
  • Paneth cell

  • yolk sac

  • primitive streak

  • endothelial cell of lymphatic vessel

  • motor neuron

  • fossa

  • blastocyst

  • Ileal epithelium

  • condyle

  • blastocyst
More reference expression data
BioGPS


More reference expression data
Gene ontology
Molecular function
  • methyltransferase activity
  • transferase activity
  • diphthine synthase activity
Cellular component
  • cytosol
  • cellular component
Biological process
  • methylation
  • metabolism
  • peptidyl-diphthamide biosynthetic process from peptidyl-histidine
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

51611

69740

Ensembl

ENSG00000117543

ENSMUSG00000033554

UniProt

Q9H2P9

Q9CWQ0

RefSeq (mRNA)

NM_001077394
NM_001077395
NM_015958

NM_027193

RefSeq (protein)

NP_001070862
NP_001070863
NP_057042

NP_081469

Location (UCSC)Chr 1: 100.99 – 101.03 MbChr 3: 115.89 – 115.93 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Diphthine synthase is an enzyme that in humans is encoded by the DPH5 gene.[5][6]

This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis.

The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene.[6]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000117543 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000033554 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Liu S, Milne GT, Kuremsky JG, Fink GR, Leppla SH (Oct 2004). "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2". Mol Cell Biol. 24 (21): 9487–97. doi:10.1128/MCB.24.21.9487-9497.2004. PMC 522255. PMID 15485916.
  6. ^ a b "Entrez Gene: DPH5 DPH5 homolog (S. cerevisiae)".

Further reading

  • Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
  • Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
  • Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
  • Dias Neto E, Correa RG, Verjovski-Almeida S, et al. (2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags". Proc. Natl. Acad. Sci. U.S.A. 97 (7): 3491–6. Bibcode:2000PNAS...97.3491D. doi:10.1073/pnas.97.7.3491. PMC 16267. PMID 10737800.
  • Lai CH, Chou CY, Ch'ang LY, et al. (2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". Genome Res. 10 (5): 703–13. doi:10.1101/gr.10.5.703. PMC 310876. PMID 10810093.
  • Hu RM, Han ZG, Song HD, et al. (2000). "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning". Proc. Natl. Acad. Sci. U.S.A. 97 (17): 9543–8. Bibcode:2000PNAS...97.9543H. doi:10.1073/pnas.160270997. PMC 16901. PMID 10931946.
  • Zhang QH, Ye M, Wu XY, et al. (2001). "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells". Genome Res. 10 (10): 1546–60. doi:10.1101/gr.140200. PMC 310934. PMID 11042152.
  • Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
  • Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
  • Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.


  • v
  • t
  • e